High throughput Sequencing

This is where our journey begins. Get to know our business and what we do, and how we're committed to quality and great service. Join us as we grow and succeed together. We're glad you're here to be a part of our story.

High throughput Sequencing

This is where our journey begins. Get to know our business and what we do, and how we're committed to quality and great service. Join us as we grow and succeed together. We're glad you're here to be a part of our story.

High throughput Sequencing

This is where our journey begins. Get to know our business and what we do, and how we're committed to quality and great service. Join us as we grow and succeed together. We're glad you're here to be a part of our story.

Targetted Gene Expression & Automated DNA Sequencing: A synopsis

In 1995, I set up a DNA sequencing core facility at the University of Manchester, based on ABI 377 Sanger sequencing technology. This involved production sequencing for a plethora of projects within Manchester laboratories, culminating in acknowledged contributions on peer reviewed papers and input into a Text book on DNA sequencing, circa 1994. I also provided in person and tutorial technical troubleshooting advice to facility clients and, on the strength of my performance, was invited by academics in Manchester to provide lectures to MSc students about DNA sequencing technology and, in addition, organise and run a 2 day practical workshop on DNA sequencing as a module on the MRes course.  

On the strength of that experience, in 2000, I was invited to participate in production sequencing, in a bespoke position, in a public consortium laboratory associated with the Human Genome project, at AECOM, Yeshiva University: Specifically, the Harvard partners health care  Center for Genetics & Genomics, linked to Mass General Brigham and the Department of Genetics . At that time, I was the first person to be hired from abroad without a PhD into the Molecular Genetics Department at AECOM.

By way of example and regarding my time in the sequencing facility at Manchester:

  1. Automated DNA Sequencing.
  2. Targeted genomic Sequencing in in vitro models of hypertension.
  3. Targeted genomic sequencing of MHC allotyes and connection to  in vitro models of auto immunity.
  4. Examples of genomic sequencing data from projects linked to the ABI 377, ABI 3100 and Amersham Megabace platforms.  

During my time in the US, I was an active participant in the Association of Biomolecular Resourse Facilities (ABRF),   including presenting posters, conference abstracts and speaking at their Annual Conference in San Diego in 2001: 

Specifically, I served as a member of the ABRF DNA Sequencing Forum and played an active role in studies which evaluated (then) current DNA technologies and how they performed in the field. Some of these studies were presented as abstracts and posters @ ABRF 2001 and published in Journal of Biomolecular Techniques. 

Examples of my DNA sequencing work, in addition to ABRF Poster publications are provided in my Weebly profile.

Project Sequencing Manchester University 

This publication on DNA sequencing features data figures supplied by me, generated on the ABI 377 at the university of Manchester. The Electropherograms were derived from PCR products which mined canine DNA for different breed specific haplotypes 

Published Book

This publication on DNA sequencing features data figures supplied by me, generated on the ABI 377 at the university of Manchester.

Project Sequencing Manchester Uni.

A paper in which I contributed to sequencing and contig building linked to candidate genes associated with biofilm Quorum sensing. I performed this work during my time at Manchester  circa 1997-2000.

ABRF 2001 Poster

A poster displayed at ABRF 2001 trade show and derived from data compiled by the DSRG working group, of which I was a member and to which I contributed.

Targetted Gene Expression & Automated DNA Sequencing: Sequencing QTLs linked to hypertension to identify causative SNPs

 

From 2002 until 2005, I was involved in a targetted sequencing project, designed to elucidate SNPs linked to hypertension, based on a rat genetic (congenic) model. Specifically, I targetted eQTL's with primers and both amplified and sequenced cDNA species in congenic hypertensive and normotensive rat models, attempting to discern differences in SNP indexed haplotypes, linked to these QTLs. The work culminated in identification of salt regulating regulating candidate genes and was reported at numerous conferences and in press.

In addition, I participated in other sequencing and genomic related projects in CVS at Leicester, including PCR genotyping of congenic mice used as models of hypertension.

Spondin 1 Paper

A paper in which a candidate gene for hypertension was identified in a rat congenic model, engendering an introgressed eQTL. I identified haplotype SNPs by DNA sequencing. Done by me during my time in CVS at Glenfield, circa 2002-2005.

Genomic mRNA paper

A paper in which different mRNA species of genes were investigated in genomic sweeps. Data provided by me was based on sequencing of specific cDNA species (RT PCR). Done by me during my time in CVS at Glenfield, circa 2002-2005.